FAQ - General Questions

Do I need prior experience with LC-MS/MS to use the facility? 

No. We offer both self-service (for trained users) and staff-run services. If you are not trained, our staff can run your samples and assist with method development and data acquisition.  

Do I need training to operate the instrument myself? 

Yes. Only authorized and trained users are permitted to operate the LC-MS system independently. Training must be completed through the HEAL Service Center, and users may only operate within the scope of that training.  

Can I let someone else run samples under my account? 

No. Instrument access is strictly limited to authorized users. Allowing untrained or unauthorized individuals to use the instrument under your account is not permitted.  

How long does an LC-MS run take? 

Run times vary depending on the method and sample type, but typically range from 10 minutes to 2 hours per sample. Longer or specialized methods may require additional time.  

What if I don’t know which LC-MS method is appropriate for my samples? 

No problem. We offer method development and sample preparation services tailored to your sample type. Our team will work with you to identify the most appropriate method and optimize conditions for your analysis.  

How can I get pricing or rates for using the LC-MS? 

For current rates and billing information, please contact us at inhealt@lehigh.edu or abs421@lehigh.edu.  

Does the HEAL Service Center provide columns, reagents, and supplies? 

Yes. We provide access to columns, solvents, reagents, and other consumables required for sample preparation and analysis. These materials will be charged to the user based on usage.  

Can I just drop off my samples and receive results? 

Yes. We offer a fully staff-run service, where you can submit your samples and our team will handle preparation (if needed), data acquisition, and initial processing.  

Do you provide help with data analysis? 

Yes. We can assist with data processing and interpretation, depending on the project scope. Please contact us in advance to discuss your analysis needs.  

Can I submit unknown samples?

An unknown sample is one where the chemical composition, properties, or potential interactions are not understood. 

Acceptable: 

• Biological samples with known origin (e.g., cell lysates, digested proteomics samples) 
• Samples that are properly prepared and validated (e.g., confirmed digestion via SDS-PAGE) 

Not acceptable: 

• Samples with unknown chemical composition 
• Materials that may react unpredictably with solvents, columns, or the instrument 

If you are unsure, please contact the facility before submitting your samples.  

What solvents are allowed? 

Samples must be prepared in LC-MS compatible solvents, including: 

• Water 
• Methanol 
• Acetonitrile 
• Small amounts of formic acid or acetic acid are acceptable. 

Any other solvents or additives must be disclosed and approved in advance.  

What types of samples are prohibited or restricted? 

The following are not allowed without prior approval: 

• High concentrations of nonvolatile salts or buffers 
• Strong acids or bases out of acceptable pH ranges 
• Particulates or poorly dissolved samples 
• Polymers  
• Proteins 
• Reactive or unstable compounds 
• Any high molecular weight or big molecules 

Additional considerations: 

• Proteins are allowed only if properly prepared, such as enzymatically digested (e.g., trypsin digestion) and free of high molecular weight species. 
• Samples containing large or high molecular weight components (e.g., intact proteins, aggregates, or poorly characterized macromolecules) may not be accepted unless reviewed and approved in advance. 
• Additionally, radioactive, biohazardous, or infectious materials are not permitted on this instrument.  

How should I prepare my samples? 

To ensure instrument safety and data quality: 

• Remove particulates (0.22 µm filtration recommended) 
• Ensure samples are fully dissolved 
• Keep protein concentration ≤ 0.5 mg/mL (for proteomics samples) 
• Use appropriate vials and clearly label all samples 
• Improper sample preparation may result in rejection or poor data quality.  

What happens if my sample damages the instrument? 

Users’ Lab are financially responsible for any damage, contamination, or service costs resulting from improper sample submission or misuse.  

How do I schedule instrument time? 

All instrument usage must be scheduled in advance through inhealt@lehigh.edu or abs421@lehigh.edu.  

Users must: 

• Use only their reserved time slot 
• Cancel at least 48 hours in advance if needed 
• Ensure runs finish within the reserved time unless approved otherwise  

What should I do if something goes wrong during a run? 

If you observe issues such as: 

• Pressure spikes 
• Leaks 
• Unusual noises 
• Signal instability

Stop the run (if safe) and contact HEAL staff immediately. Do not attempt repairs yourself.  

How long is my data stored? 

The facility stores raw data for up to 6 months. After that, data may be permanently deleted. Users are responsible for downloading and backing up their data immediately after acquisition.  

Who is responsible for compliance and safety? 

All users must follow: 

• HEAL Service Center policies 
• Institutional safety guidelines 
• Failure to comply may result in loss of instrument access.